Time-lapse Imaging of Mouse Macrophage Chemotaxis
نویسندگان
چکیده
منابع مشابه
Time-Lapse Microscopy Imaging
INTRODUCTION By their very nature, biological systems are dynamic, and a proper understanding of the cellular and molecular processes underlying living organisms and how to manipulate them is a prerequisite to combat diseases and improve human health care. One of the major challenges of current biomedical research, therefore, is to unravel not just the spatial organization of these complex syst...
متن کاملTime-Lapse Imaging
By their very nature, biological systems are dynamic, and a proper understanding of the cellular and molecular processes underlying living organisms and how to manipulate them is a prerequisite to combat diseases and improve human health care. One of the major challenges of current biomedical research, therefore, is to unravel not just the spatial organization of these complex systems, but thei...
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Five mouse macrophage cell lines were tested for chemotaxis and phagocytosis. All 5 cell lines ingested sheep red cells coated with rabbit IgG anti-Forssman antibody and exhibited chemotaxis to endotoxin-activated mouse serum (EAMS) and lymphocyte-derived chemotactic factor. Two cell lines were tested for chemotaxis to f-Met-Leu-Phe and neither responded. Four of the cell lines (RAW264, RAW309C...
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Osteoblastic mineralization occurs during the early stages of bone formation. During this mineralization, hydroxyapatite (HA), a major component of bone, is synthesized, generating hard tissue. Many of the mechanisms driving biomineralization remain unclear because the traditional biochemical assays used to investigate them are destructive techniques incompatible with viable cells. To determine...
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RATIONALE High-resolution imaging of the heart in vivo is challenging owing to the difficulty in accessing the heart and the tissue motion caused by the heartbeat. OBJECTIVE Here, we describe a suction-assisted endoscope for visualizing fluorescently labeled cells and vessels in the beating heart tissue through a small incision made in the intercostal space. METHODS AND RESULTS A suction tu...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2020
ISSN: 1940-087X
DOI: 10.3791/60750